Phytochemical and preclinical evaluation of herbal formulation as a potent health supplement

Rasika Rangari, PratikDhokne, Yogita Charde, Snehal Shrivastav, Satyendra Prasad

Shree Sainath College of Pharmacy, Dawalameti, Nagpur, Maharsatra, India

Refer this article

Rasika Rangari, Pratik Dhokne, Yogita Charde, Snehal Shrivastav, Satyendra Prasad. Phytochemical and Preclinical Evaluation of Herbal Formulation as a Potent Health Supplement. Journal of therapeutic innovation, September - October 2023, V 1 - I 2, Pages - 0038 – 0044. Doi: https://doi.org/10.55522/ijti.V1I2.0012.

ABSTRACT

The present study was carried out with the objective to perform Quality control profiling of Polyherbal formulation in the form of capsules and to evaluate its potential for weight gain and cognitive functions.The formulation was the combination of different plants (Spirulina, Ashwagandha, Brahmi, Gokhru, Shankhpushpi, Mahua, Shingada, and Bael) that are traditionally used for anti-oxidant and immunomodulatory activity and also reported for a health supplement. The parts of different plants and roots were separately dried, powdered and then extracted to evaluate various physicochemical, phytochemical and pharmaceutical parameters. Preclinical evaluation of herbal formulation was done on Swiss Albino Mice which were randomly divided into 6 groups, with 6 in each group. First group served as control, Second group was treated with Standard Protein Powder the rest four groups had doses 5, 10mg/kg of body weight for short term and 15, 20mg/kg of body weight for long term respectively. The animals were kept under observation for a period of 21 days, after 21 days, the animals were sacrificed and vital organs were dissected, weighed and samples of the tissues were fixed for histological examination. The physicochemical and pharmaceutical parameters were within the prescribed limits per standard values mentioned in API. Phytochemical screening shows alkaloids, flavonoids, tannins, alkaloids; saponin and carbohydrates found to be in considerable amounts in polyherbal formulation and the total polyphenol was found to be absent. The HPLC analysis confirmed the presence of C-Phycocyanin in formulation, which was found to be 7.36% w/w. The polyherbal formulation of health supplement was found to significantly increase in the animals treated with the control, which was quite comparable to standard C-Phycocyanin.

Keywords:

Polyherbal formulation, Physicochemical, Phytochemical, High-Performance Liquid Chromatography, C-Phycocyanin.